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KMID : 0380020050200030228
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 3 p.228 ~ p.232
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Differential Inhibitory Effect of Artemisia Extract between Staphylococcus aureus and vaginal Lactobacillus spp.
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Chung Hyun-Soo
Cha Min-Kyung
Kwon Yoon-Jung
So Jae-Seong
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Abstract
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In this study, Artemisia mongolica fischer extract was examined for its possible differential inhibitory activity against pathogenic bacteria including Staphylococcus aureus and Lactobacillus spp. isolated from women’s vagina. First, seven lactobacillus spp. were selected based on their in vitro high anti-staphylococcal activity. Various samples extracted using different concentrations of organic solvents (acetone, ethanol, methanol) were examined for optimal anti-staphylococal activity. When the Artemisia extract obtained using solvents was added to the cell suspension at (vol/vol), Lactobacillus sp. KLB 224 maintained its viability for 48 hr, whereas S. aureus was completely inactivated showing differential antimicrobial activity of the extract. Using scanning electron microscopy the effect of the extract on the cell morphology was observed: S. aureus showed markedly distorted cell morphology while Lactobacillus sp. KLB 224 appeared to remain intact.
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KEYWORD
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Artemisia extract, S. aureus, Lactobacillus spp.
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